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Publikationen

  • A. Harris, M. Wagner, D. Du, S. Raschka, L.-M. Nentwig, H. Gohlke, S. H. J. Smits, B. F. Luisi & L. Schmitt (2021) Structure and efflux mechanism of the yeast pleiotropic drug resistance transporter Pdr5 Nature Commun, in press.
  • C. Gertzen, H. Gohlke, D. Häussinger, D. Herebian, R. Kubitz, E. Mayatepek, V. Keitel, L. Schmitt (2021) The many facets of bile acids in the physiology and pathophysiology of the human liver, Biol. Chem., in press.
  • M. Prescher, M. Bonus, J. Stindt, V. Keitel-Anselmino, S. H. J. Smits, H. Gohlke, L. Schmitt (2021) Evidence for a credit-card-swipe mechanism in the human PC-floppase ABCB4, Structure, in press.

  • T. Kroll, S. H. J. Smits, L. Schmitt (2021) Monomeric bile acids modulate the ATPase activity of detergent solubilized ABCB4/MDR3, J. Lip. Res., in press.    

  • M. Lagedroste, S. H. J. Smits, L. Schmitt (2021) Importance of the leader peptide sequence on the lanthipeptide secretion level, FEBS J, in press.

  • P. Perez-Garcia, S. Kobus, C. G. W. Gertzen, A. Hoeppner, N. Holzscheck, C. H. Strunk,  H. Huber, H. Gohlke, K.-E. Jaeger, F. Kovacic, S. H. J. Smits, W. R. Streit, J. Chow (2021) The structure of a promiscuous ancestral enzyme unveils protein variable regions of diverse metallo-beta-lactamases, Comm Biol, DOI: 10.1038/s42003-021-01671-8

  • S. Maaß, J. Bartel, P. A. Mücke, R. Schlüter, T. Sura, J. Zaschke-Kriesche, S. H. J. Smits, D. Becher (2021) Proteomic Adaptation of Clostridioides difficile to Treatment with the Antimicrobial Peptide Nisin, Cells, in press.

  • L. Schäfer, C. Meinert-Berning, S. Kobus, A. Höppner, S. H. J. Smits, A. Steinbüchel (2021) Crystal structure of the sugar acid binding protein CxaP from a TRAP transporter in Advenella mimigardefordensis strain DPN7T, FEBS J, in press.

  • B.-N. Nguyen, F. Tieves, T. Rohr, H. Wobst, F. S. Schöpf, J. D. Montoya Solano, J. Schneider, J. Stock, A. Uhde, T. Kalthoff, K.-E. Jaeger, L. Schmitt, C. Schwarz (2021) Numaswitch – an efficient high-titer expression platform to produce peptides and small proteins, Appl. Microbiol. Biotechol.  express, in press.

  • M. Lagedroste, J. Reiner, V. Knospe, S. H. J. Smits & L. Schmitt (2020) A structural view on the maturation of lanthipeptides Front. Microbiol. In press.

  • K. Graefe & L. Schmitt (2020) The ABC transporter G subfamily of Arabidopsis thaliana J. Exp. Bot. in press.

  • M. Lagedroste, J. Reiners, S. H. J. Smits & L. Schmitt (2020) Impact of the nisin modification machinery on the transport kinetics of NisT Sci. Reports in press.

  • M. Montalbán-López, T. A. Scott, S. Ramesh, I. R. Rahman, A. van Heel, J. H. Viel, V. Bandarian, E. Dittmann, O. Genilloud, Y. Goto, M. José Grande Burgos, C. Hill, S. Kim, J. Koehnke, J. A. Latham, A. J. Link, B. Martínez, S. K. Nair, Y. Nicolet, S. Rebuffat. H.-G. Sahl, D. Sareen, E. W. Schmidt, L. Schmitt, K. Severinov, R. D. Süssmuth, A. W. Truman, H. Wang, J.-K. Wenig, G. P. van Wezel, Q. Zhang, J. Zhong. J. Piel, D. A. Mitchell, O. P. Kuipers & W. A. van der Donk (2020) New developments in RiPP discovery, enzymology and engineering Nat. Prod. Reviews in press.

  • C. Thomas, S. G. Aller, K. Beis, E. P. Carpenter, G. Chang, L. Chen, E. Dassa, M. Dean, F. Duong Van Hoa, D. Ekiert, R. Ford, R. Gaudet, X. Gong, I. B. Holland, Y. Huang, D. K. Kahne, H. Kato, V. Koronakis, C. M. Koth, Y. Lee, O. Lewinson, R. Lill, E. Martinoia, S. Murakami, H. W. Pinkett, B. Poolman, D. Rosenbaum, B. Sarkadi, L. Schmitt, E. Schneider, Y. Shi, S.-L. Shyng, D. J. Slotboom, E. Tajkhorshid, D. P. Tieleman, K. Ueda, A. Váradi, P.-C. Wen, N. Yan, P. Zhang, H. Zheng, J. Zimmer & R. Tampé (2020) Structural and functional diversity calls for a new classification of ABC transporters FEBS Lett. In press.

  • M. Prescher, S. H: J. Smits & L. Schmitt (2020) Stimulation of ABCB4 /MDR3 ATPase activity requires an intact phosphatidylcholine lipid J. Lip. Res.  In press.

  • J. Reiners, M. Lagedroste, J. Gottstein, E. T. Adeniyi, R. Kalschuer, G. Poschmann, K. Stühler, S. H: J. Smits & L. Schmitt (2020) Insights in the antimicrobial potential of the natural nisin variant nisin H Front. Microbiol. In press.

  • S. H. J. Smits, L. Schmitt & K. Beis (2020) Self-immunity to antibacterial peptides by ABC transporters FEBS Lett. In press.

  • Z. M. Al-Majdoub, B. Achour, N. Couto, M. Howard, Y. Elmorsi, D. Scotcher, S. Alrubia, E. El-Khateeb, A.-M. Vasilogianni, N. Alohali, S. Neuhoff, L. Schmitt, A. Rostami-Hodjegan & J. Barber (2020) Abundance of ABC Transporters in Human Liver, Gut, Kidney, Brain and Skin: A Mass Spectrometry-Based Proteomic Study FEBS Lett. In press.

  • T. Stockner, R. Gradisch & L. Schmitt (2020) The role of the degenerated site in type I ABC exporters FEBS Lett in press.

  • T. Kroll, M. Prescher, S. H. J. Smits & L. Schmitt (2020) Structure and function of hepatobiliary ABC transporters Chem. Rev. in press.

  • M. Bäumers, K. Ruhnau, T. Breuer, H. Pannen, A. Kniebel, B. Göhrlich, S. Hänsch, S. Weidtkamp-Peters, L. Schmitt & T. Klein (2020) Lethal (2) giant discs(Lgd)/CC2D1 is required for the full activity of the ESCRT machinery BMC Biology in press.

  • Hoeppner A., Bollinger A., Kobus S., Thies S., Coscolín C., Ferrer M., Jaeger K.E., Smits S.H. (2020) Crystal structures of a novel family IV esterase in free and substrate-bound form (accepted in FEBS Journal)

  •  Perez-Garcia, P., Kobus, S., Gertzen, C.G.W., Hoeppner, A., Holzscheck, N., Strunk C.H., Huber, H., Gohlke, H., Jaeger, K.-E., Kovacic, F., Smits, S.H., Streit, W.R., Chow, J. (2020) The structure of a promiscuous ancestral enzyme unveils protein variable regions of diverse metallo-beta-lactamases (accepted in Comm Biol)
  • Reiners J., Lagedroste M., Gottstein J., Adeniyi E.T., Kalscheuer R., Poschmann G, Stühler K., Smits S.H., Schmitt L. (2020) Insights in the antimicrobial potential of the natural nisin variant nisin H (accepted in Frontiers in Microbiology)
  • Furtmann F., Porta N., Hoang D.T., Reiners J., Schumacher J., Gottstein  J., Gohlke H., Smits S.H.J. (2020) Characterization of the Nucleotide-Binding Domain NsrF from the BceAB-type ABC-Transporter NsrFP from the Human Pathogen Streptococcus agalactiae Sci. Rep. (1):15208. doi: 10.1038/s41598-020-72237-7.
  • Prescher M., Smits S.H.J., Schmitt L. (2020) Molecular determinants of substrate recognition by the human lipid floppase MDR3/ABCB4 Journal of lipid research doi:10.1194/jlr.RA120000889
  • Hermann L., Mais C.N. Czech L., Smits S.H., Bange G., Bremer E. (2020) The Ups and Downs of Ectoine: Structural Enzymology of a Major Microbial Stress Protectant and Versatile Nutrient accepted in Biol Chem.
  • Czech L., Hoeppner A., Smits S.H., Bremer E. (2020) Ectoine synthase: an iron-dependent member of the cupin superfamily Encyclopedia  of Inorganic and Bioinorganic Chemistry DOI: 10.1002/9781119951438.eibc2742.
  • Mulnaes, D., Porta, N., Clemens, R., Apanasenko, I., Reiners, J., Gremer, L., Neudecker,  P., Smits, S.H.J., Gohlke, H. (2020) TopModel: Template-based protein structure prediction at low sequence identity using top-down consensus and deep neural networks. JCTC 16 (3), 1953
  •  Richter A.A., Kobus S., Czech L., Hoeppner A.,  Zarzycki J.Erb T.J., Lauterbach L., Dickschat J.S., Bremer E., Smits S.H. (2020) Making the versatile stress-protectant and chemical chaperone ectoine: architecture of the EctA diaminobutyrate acetyltransferase active site. JBC295 (9), 2822.
  •  Bollinger A, Thies S, Knieps-Grünhagen E., Gertzen C.W., Kobus S., Hoeppner A, Ferrer M., Gohlke H., Smits S.H., Jaeger K.E. (2020) A novel polyester hydrolase from the marine bacterium Pseudomonas aestusnigri - structural and functional insights  Front Life Sci 11, 114.

  •  M. Lagedroste, J. Reiners, S. H. J. Smits & L. Schmitt (2019) Systematic characterization of position one variants within the lantibiotic nisin Sci Reports 9 935.
  •   I. Dreyer, O. Spitz, K. Kanonenberg, K. Montag, M. Handrich, S. Ahmad, S. Schott-Verdugo, C. Navarro-Retamal, M. E. Rubio-Meléndez, J. L. Gomez-Porras, J. Riedelsberger, M. A. Molina-Montenegro, A. Succurro, A. Zuccaro, S. B. Gould, P. Bauer, L. Schmitt & H. Gohlke (2019) Nutrient exchange in arbuscular mycorrhizal symbiosis from a thermodynamic point of view New Phytologist 222 1043- 53.
  • K. Gräfe, K. Shanmugarajah, T. Zobel, S. Weidtkamp-Peters, D. Kleinschrodt, S. H.J. Smits & L. Schmitt (2019) Cloning and expression of selected ABC transporters from the Arabidopsis thaliana ABCG family in Pichia pastoris Plos ONE 14 e0211156.
  •  O. Spitz, I. N. Erenburg, T. Beer, K. Kanonenberg, I. B. Holland & L. Schmitt (2019) Type I secretion systems – One mechanism for all? Microbiol Spectrum 7:PSIB-0003-2018.
  •   M. Prescher, T. Kroll & L. Schmitt (2019) ABCB4 / MDR3 in Health and Disease – At the Crossroads of Biochemistry and Medicine Biol Chem 10 1245- 59.
  • P. Schwinges, S. Pariyar, F. Jakob, M. Rahimi, L. Apitius, M. Hunsche, L. Schmitt, G. Noga, C. Langenbach, U. Schwaneberg & U. Conrath (2019) A bifunctional dermaseptin-thanatin dipeptide functionalizes crop surface for sustainable pest management Green Chem. 21 2316- 25.
  • J. Sachs, K. Döhl, A. Weber, M. Bonus, F. Blesse, E. Fleischer, A. Klinger, H. Gohlke, J. Pietruszka, L. Schmitt & N. Teusch (2019) Novel 3,4-Dihydroisocoumarins Inhibit Human P-gp and BCRP in Multidrug Resistant Tumors and Demonstrate Substrate Inhibition of Yeast Pdr5 Front. Pharmacol. doi: 10.3389/fphar.2019.00400.
  •  M. Wagner, S.H.J. Smits & L. Schmitt (2019) In vitro NTPase activity of highly purified Pdr5, a major yeast ABC multidrug transporter Sci Reports 9 7761.
  • K. Kanonenberg. S.H.J. Smits & L. Schmitt (2019) Functional Reconstitution of HlyB, a Type I Secretion ABC Transporter, in Saposin-A Nanoparticles Sci Reports 9 8436.
  • N. Arya, H. Rahman, A. Rudrow, M. Wagner2, L. Schmitt, S. V. Ambudkar & J. Golin (2019) An A666G mutation in transmembrane-helix 5 of the yeast multidrug transporter Pdr5 increases drug efflux by enhancing cooperativity between transport sites Mol. Microbiol. 4 1131- 44..
  • K. Shanmugarajah, N. Linka, K. Gräfe, S. H.J. Smits, A. P.M. Weber, J. Zeier & L. Schmitt (2019) ABCG1 contributes to suberin formation in Arabidopsis thaliana roots Sci. Reports 9 11381.
  • K. Kanonenberg, J. Royes, A. Kedrov, G. Poschmann, F. Angius, A. Solgadi, O. Spitz, D. Kleinschrodt, K. Stühler, B. Miroux & L. Schmitt (2019) Shaping the lipid composition of bacterial membranes for membrane protein production. Micro. Cell. Fac. 1 131.

  •  N. Ihling, A. Uhde, R. Scholz, C. Schwarz, L. Schmitt & J. Büchs (2019) Scale-up of a Type I Secretion System in  E. coli using a defined mineral medium Biotechnol. Progr. e2911.

  •  L. Legewie, J. Loschwitz, M. Prescher, N. Steffens, X. Wang, S. H. J. Smits, L. Schmitt, B. Strodel, D. Degrandi & K. Pfeffer (2019) Biochemical and structural characterization of murine GBP7, a guanylate binding protein with an unconventional C-terminal tail Biochem J. 21 3161- 82.

  • Czech L., Wilcken S., Czech O., Linne U., Brauner J., Smits S.H., Galinski E.A., Bremer E. (2019) Exploiting substrate promiscuity of ectoine hydroxylase for regio-and stereoselective modification of homoectoine Frontiers in Microbiology 10:2745. doi: 10.3389/fmicb.2019.02745.

  • Richter A.A., Mais C-N., Czech L., Geyer K., Hoeppner, A. Smits S.H., Erb T.J.,Bange G., Bremer E. (2019)  Biosynthesis of the stress-protectant and chemical chaperon ectoine: biochemistry of the transaminase EctB Frontiers in Microbiology 10:2811. doi: 10.3389/fmicb.2019.02811

  • Porta, N., Zaschke-Kriesche, J., Frieg, B., Gopalswamy, M., Zivkovic, A., Etzkorn, M., Stark, H., Smits S.H., Gohlke, H. (2019)  Small-molecule inhibitors of nisin resistance protein NSR from the human pathogen Streptococcus agalactiae. Journal Bioorganic & Medicinal Chemistry 27(20):115079.

  • Shanmugarajah K., Linka N., Gräfe K., Smits S.H., Weber A.P., Zeier J. and Schmitt L. (2019) ABCG1 is involved in suberin barrier formation in Arabidopsis thaliana Scientific Reports 9(1):11381.

  • Zaschke-Kriesche J., Reiners J., Lagedroste M., Smits S.H. (2019) Influence of nisin hinge-region variants on immunity and resistance proteins Bioorganic & Medicinal Chemistry 27(17):3947-3953.

  • Zaschke-Kriesche J., Behrmann L.V., Reiners J., Lagedroste M., Gröner Y., Kalscheuer R., Smits S.H. (2019) Bypassing lantibiotic resistance by an effective nisin derivative Bioorganic and Medicinal Chemistry ;27(15):3454-3462.

  • Wullich S.C., Kobus S., Smits S.H., Fetzner S. (2019) Structural basis for recognition and ring-cleavage of the Pseudomonas quinolone signal (PQS) by a mycobacterial dioxygenase of the α/β-hydrolase fold family Journal of Structural biology 207(3):287-294.

  • Heilers J-H, Reiners J., Heller E.M., Smits S.H., van der Does C. (2019) DNA processing by the MOBH family relaxase TraI encoded within the gonococcal genetic island accepted  in Nucleic Acid Research  5;47(15):8136-8153.

  • Wagner M., Smits S.H.J., Schmitt L. (2019) In vitro NTPase activity of the yeast ABC transporter Pdr5 Scientific Reports 9:7761.

  • Weber J., Petrović D., Strodel B., Smits S.H.J., Kolkenbrocka S., Jaeger K.E., Leggewiea C., (2019) Carbohydrate-binding modules as strong polyethylene terephthalate binders: design and mechanical study 2019 Applied Microbiology and Biotechnology doi: 10.1007/s00253-019-09760-9.

  • Kobus S., Pérez-García P., Hoeppner A., Holzscheck N., Kovacic F., Chow J., Jaeger K.E., Streit W.R., Smits S.H. (2019) Cloning, expression, purification and preliminary X-ray analysis of Igni18, a novel metallo‑hydrolase from the hyperthermophilic archaeon Ignicoccus hospitalis KIN4/I Acta F  Apr 1;75(Pt 4):307-311. doi: 10.1107/S2053230X19002851.

  • Czech L., Höppner A., Kobus S., Seubert A., Riclea R., Dickschat J.S., Heider J., Smits S.H., and Bremer E. (2019)  Illuminating the catalytic core of ectoine synthase through structural and biochemical analysis Scientific Reports 9:364 | DOI:10.1038/s41598-018-36247-w.

  • S. Khosa, R. Scholz, C. Schwarz, M. Trilling, H. Hengel, K.-E. Jaeger, S. H. J. Smits & L. Schmitt (2018) An A/U-rich enhancer region is required for high-level protein secretion through the HlyA Type I secretion system Appl. Environm. Microbiol. 84 e01163- 17.

  • T. Hoffmann, B. Warmbold, S. H. J. Smits, B. Tschapek, S. Ronzheimer, A. Bashir, C. Chen, A. Rolbetzki, M. Pittelkow, M. Jebbar, A. Seubert, L. Schmitt & E. Bremer (2018) Arsenobetaine: an ecophysiologically important organoarsenical confers cytoprotection against osmotic stress and growth temperature extremes Environ. Microbiol. 20 305- 323 .

  • K. Kanonenberg, O. Spitz, I. Erenburg, T. Beer & L. Schmitt (2018) Type I Secretion System – It takes three and a substrate FEMS Microbiol. Lett. 365 fny094.

  • S. Peherstorfer, H. H. Brewitz, A. A. P. George, A. Wißbrock, J. M. Adam, L. Schmitt & D. Imhof (2018) Insights into mechanism and functional consequences of heme binding to hemolysin-activating lysine acyltransferase HlyC from Escherichia coli Biochim. Biophys. Acta 1862 1964- 1972.

  •  L. Schmitt (2018) Vitamin B12 import is all about timing Nature Chem. Biol. 14 640- 41.

  •  K. Tanabe, M. Bonus, S. Tomiyama, K. Miyoshi, M. Nagi, K. Niimi, A Chindamporn, H. Gohlke, L. Schmitt, R. D. Cannon, M. Niimi & E. Lamping (2018) FK506 resistance of Saccharomyces cerevisiae Pdr5 and Candida albicans Cdr1 involves mutations in the transmembrane domains and extracellular loops Antimicrob. Agent. Chemotherap. 63 e01146- 18.

  • Czech L., Hermann L., Stöveken N., Richter AA., Höppner A., Smits, SH, Heider J., and Bremer E. (2018) Role of the extremolytes ectoine and hydroxyectoine as both stress protectants and nutrients: genetics, phylogenomics, biochemistry, and structural analysis Gene 9(4) doi: 10.3390/genes9040177.

  • Clemens, R., Zaschke-Kriesche, J., Khosa, S., and Smits, S.H.J. (2018). Insight into Two ABC Transporter Families Involved in Lantibiotic Resistance. Frontiers in Molecular Biosciences 4. doi: 10.3389/fmolb.2017.00091.

  • J. Reiners, A. Abts, R. Clemens, S. H. J. Smits & L. Schmitt (2017) Stoichiometry and structure of a lantibiotic maturation complex   Sci Reports 7 42163.

  • A. Weber, K. Döhl, J. Sachs, A. C. M. Nordschild, D. Schröder, A. Kulik, T. Fischer, L. Schmitt, N. Teusch & J. Pietruszka (2017) Synthesis and Cytotoxic Activities of Goniothalamins and Derivatives Bioorg. & Med. Chem.  16 31101-31104.

  • L. Teichmann, C. Chen, T. Hoffmann, S. H. J. Smits, L. Schmitt & E. Bremer (2017) From substrate specificity to promiscuity: hybrid ABC transporters for osmoprotectants Mol. Microbiol. 104 761- 780.

  •  P. Ellinger, J. Stindt, C. Dröge, K. Sattler, C. Stross, S. Kluge, D. Herebian, S. H J Smits, M. Burdelski, S. Schulz-Jürgensen, A. Ballauff, J. Schulte am Esch, E. Mayatepek, D. Häussinger, R. Kubitz & L. Schmitt (2017) Partial external biliary diversion in bile salt export pump deficiency: Association between outcome and mutation World J. Gastroentereology 23 5295- 5303.

  • M. Lagedroste, S. H. J. Smits & L. Schmitt (2017) Substrate specificity of the secreted nisin leader peptidase NisP Biochemistry 56 4005- 4014.

  • C. Dröge, M. Bonus, U. Baumann, C. Kindt, E.  Lainka, S. Kathemann, F. Brinkert, E. Grabhorn, E. D. Pfister, D. Wenning,. A. Fichtner, D. N. Gotthardt, K. H. Weiss, P. McKiernan, R. D. Puri, I. C. Verma, S. Kluge, H. Gohlke, L. Schmitt, R. Kubitz, D. Häussinger, V. Keitel (2017) Sequencing of FIC1, BSEP and MDR3 in a large cohort of patients with cholestasis revealed a high number of genetic variants J Hepatology 67 1253- 1257.

  • Herebian, D., Seibt, A., Smits, S.H.J., Rodenburg, R.J., Mayatepek, E., and Distelmaier, F. (2017). 4-Hydroxybenzoic acid restores CoQ10 biosynthesis in human COQ2 deficiency. Ann Clin Transl Neurol 4, 902-908. doi: 10.1002/acn3.486.

  • Reiners, J., Lagedroste, M., Ehlen, K., Leusch, S., Zaschke-Kriesche, J., and Smits, S.H.J. (2017). The N-terminal Region of Nisin Is Important for the BceAB-Type ABC Transporter NsrFP from Streptococcus agalactiae COH1. Frontiers in Microbiology 8. doi: 10.3389/fmicb.2017.01643.

  • Herebian, D., Seibt, A., Smits, S.H.J., Bunning, G., Freyer, C., Prokisch, H., Karall, D., Wredenberg, A., Wedell, A., Lopez, L.C., Mayatepek, E., and Distelmaier, F. (2017). Detection of 6-demethoxyubiquinone in CoQ10 deficiency disorders: Insights into enzyme interactions and identification of potential therapeutics. Mol Genet Metab 121, 216-223. doi: 10.1016/j.ymgme.2017.05.012.

  • Kroeger, T., Frieg, B., Zhang, T., Hansen, F.K., Marmann, A., Proksch, P., Nagel-Steger, L., Groth, G., Smits, S.H.J., and Gohlke, H. (2017). EDTA aggregates induce SYPRO orange-based fluorescence in thermal shift assay. PLoS One 12, e0177024. doi: 10.1371/journal.pone.0177024.

  • J. Stindt, S. Kluge, C. Dröge, V. Keitel, C. Stross, U. Baumann, F. Brinkert, A. Dhawan, G. Engelmann, R. Ganschow, P. Gerner, E. Grabhorn, A. S. Knisely, K. A. Nouli, I. Pukite, R. W. Sheppard, T. Ueno, L. Schmitt, C. Wiek, H. Hanenberg, D. Häussinger & R. Kubitz (2016) Bile Salt Export Pump-reactive Antibodies Form a Polyclonal, Multi-inhibitory Response in Antibody-induced BSEP Deficiency Hepatology 63 524- 537.

  • S. Khosa, A. Höppner, H. Gohlke, L. Schmitt & S. H. J. Smits (2016) Structure of the response regulator NsrR from Streptococcus agalactiae, which is involved in lantibiotic resistance PlosOne 11 e0149903.

  • T. Shekhar-Guturja, G. M. K. B. Gunaherath, E. M. K. Wijeratne, J.-P. Lambert, A. F. Averette, S. C. Lee, T. Kim, Y.-S. Bahn, F. Tripodi, R. Ammar, K. Döhl, K. Niewola-Staszkowska, L. Schmitt, R. J. Loewith, F. P. Roth, D. Sanglard, D. Andes, C. Nislow, P. Coccetti, A.-C. Gingras, J. Heitman, A. A. L. Gunatilaka & L. E. Cowen (2016) Dual Action Small Molecule Potentiates Antifungal Efficacy, Blocks the Evolution of Drug Resistance, and Renders Resistant Pathogens Responsive to Therapy via Modulation of Multidrug Efflux and TOR Signaling Nat. Chem. Biol. 12 867- .

  • S. Reimann, G. Poschmann, K. Kanonnenberg, K. Stühler, S. H. J. Smits & L. Schmitt (2016) Interdomain regulation of the ATPase activity of the ABC transporter hemolysin B from E. coli Biochem. J.  473 2471- 2483.

  • S. Przybylla, J. Stindt, D. Kleinschrodt, J. Schulte Am Esch, D. Häussinger, V. Keitel, S. H. J. Smits & L. Schmitt (2016) Analysis of the Bile Salt Export Pump (ABCB11) Interactome Employing Complementary Approaches PLOSOne 11 e0159778.

  •  M. Wagner, K. Döhl & L. Schmitt (2016) Transmitting the energy: Interdomain cross-talk in Pdr5 Biol. Chem. 398 145- 154.

  • S.E.T. Luczak, S.H.J. Smits, C. Decker, L. Nagel-Steger, L. Schmitt & J.H. Hegemann (2016) The Chlamydia pneumoniae Adhesin Pmp21 forms Oligomers with Adhesive Properties J. Biol. Chem. 291 22806- 22818.

  • M. H. H. Lenders, T. Beer, S. H. J. Smits & L. Schmitt (2016) In vivo quantification of the secretion rates of the hemolysin A Type I secretion system Sci. Reports 6 33275.

  • I. B. Holland, S. Peherstorfer, K. Kanonenberg, M. Lenders, S. Reimann & L. Schmitt (2016) Type I protein secretion, deceptively simple yet with a range of mechanistic variability across the family EcoSalPlus doi:10.1128/ecosalplus. ESP-0019-2015.

  • K. Nouri, E. K. Fansa, E. Amin, R. Dvorsky, L. Gremer, D. Willbold, L. Schmitt, D. J. Timson & M. R. Ahmadian (2016) IQGAP1 interaction with RHO family proteins revisited: Kinetic and equilibrium evidence for multiple distinct binding sites J. Biol. Chem. 291 26364-26376.

  • Khosa, S., B. Frieg, D. Mulnaes, D. Kleinschrodt, A. Hoeppner, H. Gohlke & S.H. Smits, (2016) Structural basis of lantibiotic recognition by the nisin resistance protein from Streptococcus agalactiae. Scientific reports 6: 18679.

  • Khosa, S., M. Lagedroste & S.H. Smits, (2016c) Protein Defense Systems against the Lantibiotic Nisin: Function of the Immunity Protein NisI and the Resistance Protein NSR. Frontiers in microbiology 7: 504.

  • Widderich, N., S. Kobus, A. Hoppner, R. Riclea, A. Seubert, J.S. Dickschat, J. Heider, S.H. Smits & E. Bremer, (2016) Biochemistry and Crystal Structure of Ectoine Synthase: A Metal-Containing Member of the Cupin Superfamily. PloS one 11: e0151285.

  • Widderich, N., E. Bremer & S.H.J. Smits, (2016) The Ectoine Hydroxylase: A Nonheme-Containing Iron(II) and 2-Oxoglutarate-Dependent Dioxygenase. In: Encyclopedia of Inorganic and Bioinorganic Chemistry. John Wiley & Sons, Ltd, pp.

  • Zhang, Z., Q. Gu, A.A. Jaguva Vasudevan, A. Hain, B.P. Kloke, S. Hasheminasab, D. Mulnaes, K. Sato, K. Cichutek, D. Haussinger, I.G. Bravo, S.H. Smits, H. Gohlke & C. Munk, (2016) Determinants of FIV and HIV Vif sensitivity of feline APOBEC3 restriction factors. Retrovirology 13: 46.

  • Zhang, Z., Q. Gu, A.A. Jaguva Vasudevan, M. Jeyaraj, S. Schmidt, J. Zielonka, M. Perkovic, J.O. Heckel, K. Cichutek, D. Haussinger, S.H. Smits & C. Munk, (2016) Vif Proteins from Diverse Human Immunodeficiency Virus/Simian Immunodeficiency Virus Lineages Have Distinct Binding Sites in A3C. Journal of virology 90: 10193-10208.

  • M. Kluth, J. Stindt, C. Droege, D. Linnemann, R. Kubitz & L. Schmitt (2015) A mutation within the extended X loop abolished substrate-induced ATPase activity of the human liver ABC Transporter MDR3 J Biol Chem 290 4896- 907.

  • B. Tschapek, S. H. J. Smits & L. Schmitt (2015) Analyzing the physico-chemical parameters of detergents and detergent mixture Adv Chem Engineering Sci 5 328- 337

  •  A. Pelzer, C. Schwarz, S. Smits, A. Wirtz, S. Wilhelm, L. Schmitt, H. Funken & K.-E. Jaeger (2015) Functional expression, purification and biochemical properties of subtilase SprP from Pseudomonas aeruginosa Microbiology Open 4 743- 752.

  • M. H. H. Lenders, S. Weidtkamp-Peters, D. Kleinschrodt, K.-E. Jaeger, S. H. J. Smits & L.  Schmitt (2015) Directionality of substrate translocation of the hemolysin A Type I secretion system Scientific Reports 5 12470.

  • K. Nouri, J. M. Moll, L. G. Milroy, A. Hain, R. Dvorsky, E. Amin, M. Lenders, L. Nagel-Steger, S. Howe, S. H. Smits, H. Hengel, L. Schmitt, C. Münk, L. Brunsveld & M. R. Ahmadian (2015) Biophysical characterization of nucleophosmin interactions with human immunodeficiency virus Rev and Herpes Simplex virus US11 PlosOne 10 e0143634.

  • Broy, S., C. Chen, T. Hoffmann, N.L. Brock, G. Nau-Wagner, M. Jebbar, S.H. Smits, J.S. Dickschat & E. Bremer, (2015) Abiotic stress protection by ecologically abundant dimethylsulfoniopropionate and its natural and synthetic derivatives: insights from Bacillus subtilis. Environmental microbiology 17: 2362-2378.

  • Gertzen, C.G., L. Spomer, S.H. Smits, D. Haussinger, V. Keitel & H. Gohlke, (2015) Mutational mapping of the transmembrane binding site of the G-protein coupled receptor TGR5 and binding mode prediction of TGR5 agonists. European journal of medicinal chemistry 104: 57-72.

  • Khosa, S., A. Hoeppner, D. Kleinschrodt & S.H. Smits, (2015) Overexpression, purification and crystallization of the response regulator NsrR involved in nisin resistance. Acta crystallographica. Section F, Structural biology communications 71: 1322-1326.

  • Khosa, S., A. Hoeppner, D. Kleinschrodt & S.H. Smits, (2015) Overexpression, purification, crystallization and preliminary X-ray diffraction of the nisin resistance protein from Streptococcus agalactiae. Acta crystallographica. Section F, Structural biology communications 71: 671-675.

  • Kobus, S., N. Widderich, A. Hoeppner, E. Bremer & S.H. Smits, (2015) Overproduction, crystallization and X-ray diffraction data analysis of ectoine synthase from the cold-adapted marine bacterium Sphingopyxis alaskensis. Acta crystallographica. Section F, Structural biology communications 71: 1027-1032.


  • H. C. Thakur, M. Singh, L. Nagel-Steger, J. Kremer, D. Prumbaum, E. Kalawy Fansa, H. Ezzahoin1, K. Nouri, L. Gremer, A. Abts, L. Schmitt, S. Raunser, M. R. Ahmadian & R. P. Piekorz (2014) Two functionally diverse modules of the TACC domain of the centrosomal adaptor TACC3 determine binding to the microtubule polymerase chTOG in an Aurora-A independent manner J. Biol. Chem 289 74- 88.

  •  E. Ciglia, J. Vergin, S. Reimann, S. Smits, L. Schmitt, G. Groth & H. Gohlke (2014) Resolving Hot Spots in the C-Terminal Dimerization Domain that Determine the Stability of the Molecular Chaperone Hsp90 PlosOne 9 e96031.

  • R. Gupta, P. Küppers, N. Hanekop & L. Schmitt (2014) Generating symmetry in the asymmetric ABC transporter Pdr5 from Saccharomyces cerevisiae J. Biol. Chem, 289 15272- 15279.

  • I. Gawarzewski, F. DiMaio, E. Winterer, B. Tschapek, S. H.J. Smits, J. Jose & L. Schmitt (2014) Crystal structure of the transport unit of the autotransporter adhesin involved in diffuse adherence from Escherichia coli J Struct Biol 187 20- 29. 

  • S. Thomas, P. Bakkes, S. H. J. Smits & L. Schmitt (2014) Equilibrium folding of pro-HlyA from Escherichia coli reveals a stable calcium ion dependent folding intermediate Biochim. Biophys. Acta 1844 1500- 1510.

  •  S. Thomas, S. H. J. Smits & L. Schmitt (2014) A simple in vitro acylation assay based on optimized HlyA and HlyC purification Anal. Biochem. 464 17- 23.

  • R. Gupta, P. Kueppers & L. Schmitt (2014) New examples of membrane protein expression and purification using the yeast based Pdr1-3 expression strategy J. Biotechnol. 191 158- 164.

  • AlKhatib, Z., M. Lagedroste, I. Fey, D. Kleinschrodt, A. Abts & S.H. Smits, (2014a) Lantibiotic immunity: inhibition of nisin mediated pore formation by NisI. PloS one 9: e102246.

  • AlKhatib, Z., M. Lagedroste, J. Zaschke, M. Wagner, A. Abts, I. Fey, D. Kleinschrodt & S.H. Smits, (2014b) The C-terminus of nisin is important for the ABC transporter NisFEG to confer immunity in Lactococcus lactis. MicrobiologyOpen 3: 752-763.
  • Bashir, A., T. Hoffmann, B. Kempf, X. Xie, S.H. Smits & E. Bremer, (2014) Plant-derived compatible solutes proline betaine and betonicine confer enhanced osmotic and temperature stress tolerance to Bacillus subtilis. Microbiology 160: 2283-2294.
  • Bashir, A., T. Hoffmann, S.H. Smits & E. Bremer, (2014) Dimethylglycine provides salt and temperature stress protection to Bacillus subtilis. Applied and environmental microbiology 80: 2773-2785.
  • Hoeppner, A., N. Widderich, E. Bremer & S.H. Smits, (2014) Overexpression, crystallization and preliminary X-ray crystallographic analysis of the ectoine hydroxylase from Sphingopyxis alaskensis. Acta crystallographica. Section F, Structural biology communications 70: 493-496.

  • Hoppner, A., N. Widderich, M. Lenders, E. Bremer & S.H. Smits, (2014) Crystal structure of the ectoine hydroxylase, a snapshot of the active site. The Journal of biological chemistry 289: 29570-29583.

  • Kluth M., Dohl K., Ellinger P., Przybylla S., Smits S.H. & Schmitt. L., (2014) Identification of new interaction partners of the human ABC transporter MDR3. European Journal of Medical Research 19: 1.

  • Koster, K.L., M. Sturm, D. Herebian, S.H. Smits & U. Spiekerkoetter, (2014) Functional studies of 18 heterologously expressed medium-chain acyl-CoA dehydrogenase (MCAD) variants. Journal of inherited metabolic disease 37: 917-928.

  • Thomas, S., P.J. Bakkes, S.H. Smits & L. Schmitt, (2014a) Equilibrium folding of pro-HlyA from Escherichia coli reveals a stable calcium ion dependent folding intermediate. Biochimica et biophysica acta 1844: 1500-1510.

  • Widderich, N., A. Hoppner, M. Pittelkow, J. Heider, S.H. Smits & E. Bremer, (2014) Biochemical properties of ectoine hydroxylases from extremophiles and their wider taxonomic distribution among microorganisms. PloS one 9: e93809.

  • Widderich, N., M. Pittelkow, A. Hoppner, D. Mulnaes, W. Buckel, H. Gohlke, S.H. Smits & E. Bremer, (2014) Molecular dynamics simulations and structure-guided mutagenesis provide insight into the architecture of the catalytic core of the ectoine hydroxylase. Journal of molecular biology 426: 586-600.

  • N. Infed, S. H. J. Smits, T. Dittrich, M. Braun, A. J. M. Driessen, N. Hanekop & L. Schmitt (2013) Analysis of the inhibition potential of zosuquidar derivatives on selected bacterial and fungal ABC transporters Mol Memb Biol 30 217- 227.

  • P. Ellinger, M. Kluth, J. Stindt, S. H. J. Smits & L. Schmitt (2013) Detergent Screening and Purification of the Human Liver ABC Transporters BSEP (ABCB11) and MDR3 (ABCB4) expressed in the yeast Pichia pastoris PlosOne 8 e60620.

  • P. Küppers, R. P. Gupta, J. Stindt, S. H. Smits & L. Schmitt (2013) The functional impact of a single mutation within the transmembrane domain of the multidrug ABC transporter Pdr5 Biochemistry 52 2184- 2195.

  • K. Kanonenberg, C. K. W. Schwarz & L. Schmitt (2013) Type I secreion systems – a story of appendices Res Microbiol 164 596- 604.

  • Z. Arslan, R. Wurm, O. Brener, P. Ellinger, L. Nagel-Steger, F. Oesterhelt, L. Schmitt, D. Willbold, R. Wagner, H. Gohlke, S. Smits, Sander & Ü. Pul (2013) Double-strand DNA end-binding and sliding of the toroidal CRISPR-associated protein Csn2 Nucl. Acid Res. 41 6347- 6359.

  • J. Stindt, P. Ellinger, K. Weissenberger, C. Dröge, D. Herebian, E. Mayatepek, B. Homey, S. Braun, J. Schulte am Esch, M. Horacek, A. Cambay, L. Schmitt, D. Häussinger & R. Kubitz (2013) A novel mutation within a transmembrane helix of the bile salt export pump (BSEP) with delayed development of liver cirrhosis Liver Intl. 33 1527- 1535.

  • H. Thakur, S. Madhurenda, L. Nagel-Steeger, D. Prumbach, E. Kalawy Fansa, L. Gremer, H. Ezzahoini, A. Abts, L. Schmitt, S. Rausner, R. Ahmadian & R. Piekorz (2013) Role of centrosomal adaptor proteins of the TACC family in the regulation of microtubule dynamics during mitotic cell division Biol. Chem 394 1411- 1423.

  • M. Lenders, S. Reimann, S. H. J. Smits & L. Schmitt (2013) Molecular Insights into Type I Secretion Systems Biol. Chem 394 1371- 1384.

  • A. Abts, M. Montalban-Lopez, O. P. Kuipers, S. H. J. Smits & L. Schmitt (2013) NisC binds the FxLx motif of the nisin leader peptide Biochemistry 52 5387- 5395.

  • B. Lipowicz, N. Hanekop, L. Schmitt & P. Proksch (2013) An aeroplysinin-1 specific nitrile hydratase from the marine sponge Aplysina cavernicola Marine Drugs 11 3046- 3067.

  • I. Gawarzewski, S. H.J. Smits, L. Schmitt & J. José (2013) Structural comparison of the transport units of type V secretion systems Biol. Chem 394 1385- 1398.

  • I. Gawarzewski,  B. Tschapek,  A. Hoeppner,  J. Jose,  S. Smits & L. Schmitt (2013) Purification, crystallization and preliminary X-ray crystallographic studies of the transport unit of the monomeric autotransporter AIDA-I from Escherichia coli Acta Cryst F 69 1159- 1162.

  • H. Gohlke, U. Hergert, T. Meyer, D Mulnaes, Daniel, M. K. Grieshaber, S. H. J. Smits & L. Schmitt (2013) Binding region of alanopine dehydrogenase predicted by unbiased molecular dynamics simulations of ligand diffusion J. Chem. Inform. Model 53 2493- 2498.

  • S. Thomas, I. B. Holland & L. Schmitt (2013) The Type 1 Secretion Pathway – the haemolysin system and beyond Biochim Biophys Acta Mol Cell Res 1843 1629- 1641.

  • Khosa, S., Z. AlKhatib & S.H. Smits, (2013) NSR from Streptococcus agalactiae confers resistance against nisin and is encoded by a conserved nsr operon. Biological chemistry 394: 1543-1549.

  • Vasudevan, A.A., S.H. Smits, A. Hoppner, D. Haussinger, B.W. Koenig & C. Munk, (2013) Structural features of antiviral DNA cytidine deaminases. Biological chemistry 394: 1357-1370.

  • P. Ellinger, M. Kluth, S. Pryzbylla, S. H. J. Smits & L. Schmitt (2012) Structure and function of hepatic ABC transporters Hepatobiliary transport in health and disease, DeGruyter, 23- 48.
  • N. van Oz, S. H. J. Smits, L. Schmitt & M. K. Grieshaber (2012) Thermodynamic Studies of Binary and Ternary Complex Association of Octopine Dehydrogenase Implicated with the Regulation of D-Octopine Formation in the Adductor Muscle of the Great Scallop, Pecten maximus (L.) J. Exp. Biol. 215 1515- 1522.

  • Z. Alkhatib, A. Abts, A. Mavaro, L. Schmitt & S. H. J. Smits (2012) Lantibiotics: How do producers become self-protected? J. Biotechnol. 159 145- 154.

  • C. K. W. Schwarz, C. D. Landsberg, M. H. H. Lenders, S. H. J. Smits & L. Schmitt (2012) Using an E. coli Type 1 secretion system to secrete the mammalian, intracellular protein IFABP in its active form J. Biotechnol. 159 155- 161.

  • P. Ellinger, Z. Arslan, R. Wurm, B. Tschapek, C. MacKenzie, K. Pfeffer, S. Panjikar, R. Wagner, L. Schmitt, H. Gohlke, Ü. Pul & S. H. J. Smits (2012) The crystal structure of the CRISPR-associated protein Csn2 from Streptococcus agalactiae J. Struct. Biol. 178 350- 362.

  • C. K. W. Schwarz, M. H. H. Lenders, S. H. J. Smits & L. Schmitt (2012) Secretion of slow-folding proteins by a Type 1 Secretion System Bioengineered 5 1- 4.

  • E. Kravets, D. Degrandi, S. Weitkamp-Peters, B. Ries, C. Konermann, S. Felekyan, J. M. Dargazanli5, G. J. K. Praefcke, C. A. M. Seidel, L. Schmitt, S. H. J. Smits, K. Pfeffer (2012) The GTPase activity of murine guanylate-binding protein 2 (mGBP2) controls the intracellular localization and recruitment to the parasitophorous vacuole of Toxoplasma gondii J. Biol. Chem. 287 27452- 27466.

  • T. Dzagania, G. Engelmann, D. Häussinger, L. Schmitt, C. Flechtenmacher, I. Rtskhiladze & R. Kubitz (2012) The Histidin-loop is essential for transport activity of human MDR3: A novel mutation of MDR3 in a patient with progressive familial intrahepatic cholestasis type 3. Gene 506 141- 145.

  • J. Lecher, C. K. W. Schwarz, M. Stoldt, S. H. J. Smits, D. Willbold & L. Schmitt (2012) An RTX Transporter Tethers Its Unfolded Substrate during Secretion via a Unique N-Terminal Domain. Structure 20 1- 10.

  • T. Dittrich, N. Hanekop, N. Infed, L. Schmitt & M. Braun (2012) Synthesis of 5-Oxyquinoline Derivatives for Reversal Multidrug Resistance. Beilstein J. Org. Chem. 8 1700- 1704.

  • D. Schneider & L. Schmitt (2012) Highlight: Membrane transport and beyond. Biol Chem. 393 (11), 1201-1202.

  • A. Höppner, L. Schmitt & S. H. J. Smits (2012) Proteins and their ligands: Their importance and how to obtain a crystal Crystal growth, 1- 42.

  • van Os, N., S.H. Smits, L. Schmitt & M.K. Grieshaber, (2012) Control of D-octopine formation in scallop adductor muscle as revealed through thermodynamic studies of octopine dehydrogenase. The Journal of experimental biology 215: 1515-1522.

  • T. Jumpertz, B. Tschapek, N. Infed, S. H. J. Smits, R. Ernst & L. Schmitt (2011) High throughput evaluation of the critical micelle concentration of detergents Anal. Biochem. 408 64- 70.

  • R. Gupta, P. Kueppers, L. Schmitt & R. Ernst (2011) The multidrug transporter   Pdr-5  a molecular diode? Biol. Chem. 392 53- 60.

  • J. Lecher, M. Stoldt, C. K. W. Schwarz, S. H. J. Smits, L. Schmitt & D. Willbold (2011) ¹H, ¹⁵N and ¹³C resonance assignment of the N-terminal C39 peptidase-like domain of the ABC transporter Haemolysin B (HlyB). Biomol. NMR Assign. 5 199-201.

  • C. Schwarz,   B. Tschapek,   T. Jumpertz,   S. Jenewein,   J. Lecher,   D. Willbold,   S. Panjikar,   B. Holland,   S. Smits & L. Schmitt (2011) Crystallisation and preliminary X-ray crystallographic studies of an oligomeric species of a refolded C39 peptidase-like domain of the Escherichia coli ABC transporter Haemolysin B Acta Crystallograph F 67 630-633.

  • J. Stindt, P. Ellinger, C. Stross, V. Keitel, D. Häussinger, S. H. J. Smits, R. Kubitz & L. Schmitt (2011) Heterologous Overexpression and Mutagenesis of the Human Bile Salt Export Pump (ABCB11) using DREAM (Directed REcombination-Assisted Mutagenesis) PLOSOne 6 e20562.

  • N. Infed, N. Hanekop, A. J. Driessen; S. H. J. Smits & L. Schmitt (2011) Influence of detergents on the activity of the ABC transporter LmrA BBA Biomembranes 1808 2313- 2321.

  • M. Pittelkow, B. Tschapek, S. H. J. Smits, L. Schmitt & E. Bremer (2011) The crystal structure of the substrate-binding protein OpuBC from Bacillus subtilis in complex with choline J. Mol. Biol. 411 53-67.

  • B. Tschapek, M. Pittelkow, L. Sohn-Bösser, G. Holtmann, S. H. J. Smits, H. Gohlke, E. Bremer & L. Schmitt (2011) Arg149 is involved in switching the low affinity, open state of the binding protein AfProX into its high affinity, closed state J Mol Biol 411 36-52.

  • A. Abts, A. Mavaro, J. Stindt, P. Bakkes, S. Metzger, A. J. M. Driessen, S. H. J.  Smits & L. Schmitt (2011) Easy and rapid purification of highly active nisin Int. J. Peptides 1745145.

  • A. Mavaro, A. Abts, P. Bakkes, S. H. J. Smits & L. Schmitt (2011) Substrate recognition and specificty of NisB, the lantibiotic dehydratase involved in nisin biosynthesis J. Biol. Chem. 286 30552- 30560.

  • A. Abts, C. K. H. Schwarz, B. Tschapek, S. H: J. Smits & L. Schmitt (2011) Rational and irrational approaches to convince a protein to crystallize Crystal Growth/Book 1 eds. In Tech, 497- 528.

  • Abts, A., A. Mavaro, J. Stindt, P.J. Bakkes, S. Metzger, A.J. Driessen, S.H. Smits & L. Schmitt, (2011) Easy and rapid purification of highly active nisin. International journal of peptides 2011: 175145.

  • R. Ernst, P. Kueppers, J. Stindt, K. Kuchler & L. Schmitt (2010) Multidrug efflux pumps: Substrate selection in ATP-binding cassette multidrug efflux pumps – first come first served? FEBS J. 277 540- 549.

  • R. P.-A. Berntsson, S. H. J. Smits, L. Schmitt, D.-J. Slotboom & B. Poolman (2010) A structural classification of substrate-binding proteins FEBS Lett., 584 2606- 2617.

  • K. J. Linton, K. J. Zolnerciks & L. Schmitt (2010) General introduction, structure and likely mechanism of ABC transport proteins, „ABC transporters in Physiology and Disease, 1- 28.

  • T. Jumpertz, C. Chervaux, K. Racher, M. Zouhair, M. A. Blight, I. B. Holland & L. Schmitt  (2010) Mutations affecting the extreme C-terminus of E. coli haemolysin A reduce haemolytic activity by altering the folding of the toxin Microbiology 156 2495- 2505.

  • S. H. J. Smits, T. Meyer, A. Mueller, N. van Os, M. Stoldt, D. Willbold, L. Schmitt & M. K. Grieshaber (2010) Insights into the mechanism of ligand binding to octopine dehydrogenase from Pecten maximus by NMR and crystallography PLOS One 5 e12312.

  • P. Bakkes, S. Jenewein, S. H. J. Smits, I. B. Holland & L. Schmitt (2010) The rate of folding dictates substrate secretion by the Escherichia coli haemolysin type 1 secretion system J. Biol. Chem. 24 40573-40580.

     


  • S. Jenewein, I. B. Holland  & L. Schmitt (2009) “Bacterial Type I Secretion” in Bacterial protein secretion (ed K. Woolridge), Caister Academic Press, 45- 66.

  • T. Jumpertz, I. B. Holland & L. Schmitt (2009) "ABC transporters - a smart example of a molecular machinerie" in ABC transporters in microorganisms, Caister Academic Press, 1- 34.

  • S. Smits & L. Schmitt (2009) Eine Strukturbiologie für Membranproteine Nachr. Chemie und Techn. 1 23-27.

  •  J. Lecher, M. Pittelkov, S. Zobel, J. Bursy, T. Böing, S. H. J. Smits, L. Schmitt &, E. Bremer (2009) The crystal structure of UehA in complex with ectoine - A comparison with other TRAP transporter binding proteins J. Mol. Biol. 389 58-73.

  • V. Keitel, M. Burdelski, Z. Vojnisek, L. Schmitt, D. Häussinger & R. Kubitz (2009) De novo bile salt transporter antibodies as a cause of recurrent graft failure after liver transplantation: a novel mechanism of cholestasis Hepatology 50 510- 517.

  • C. Oswald, S. H. Smits, M. Hoeing, E. Bremer & L. Schmitt (2009) Structural analysis of the choline binding protein ChoX in a semi-closed and ligand-free conformation Biol Chem 390 1163-1170.
  • C. Oswald, S. Jenewein, S. H. Smits, I. B. Holland & L. Schmitt (2008) Water-mediated protein-fluorophore interactions modulate the affinity of an ABC-ATPase/TNP-ADP complex, J. Struct. Biol. 162 85- 93.

  • R. Ernst, P. Kueppers, C. Klein, T. Schwarzmueller, K. Kuchler & L. Schmitt (2008) A mutation of the H-loop selectively affects rhodamine transport by the yeast multidrug ABC transporter Pdr5 Proc. Natl. Acad. Sci USA 105 5069-5074.

  • C. Horn, S. Jenewein, B. Tschapek, W. Bouschen, S. Metzger, E. Bremer & L. Schmitt (2008) Monitoring conformational changes during the catalytic cycle of OpuAA, the ATPase subunit of the ABC-transporter OpuA from Bacillus subtilis, Biochem. J., 412 233- 244.

  • S. H. Smits, A. Mueller, L. Schmitt & M. K. Grieshaber (2008) A structural basis for substrate and stereo selectivity in octopine dehydrogenase from Pecten maximus, J. Mol. Biol. 381 200- 211.

  • S. H. Smits, M. Höing, J. Lecher, M. Jebbar, L. Schmitt & E. Bremer (2008) The compatible solute-binding protein OpuAC from Bacillus subtilis: ligand-binding, site directed mutagenesis and crystallographic studies, J. Bacteriol. 190 5663- 5671.

  • C. Oswald, S. H. Smits, E. Bremer & L. Schmitt (2008) Microseeding – A Powerful Tool for Crystallizing Proteins in Complex with Hydrolyzable Substrates, Internat. J. Mol. Sci. 9 1131- 1141.

  • S. H. Smits, A. Mueller, M. K. Grieshaber & L. Schmitt (2008) Coenzyme- and His-tag-induced crystallization of octopine dehydrogenase. Acta Cryst. F 64 836- 839.

  • C. Oswald, S. H. J. Smits, M. Höing, L. Sohn-Bösser, L. Dupont, D. Le Rudulier, L. Schmitt & E. Bremer (2008) Crystal structures of the choline/acetylcholine substrate binding protein ChoX from Sinorhizobium meliloti in the liganded and unliganded closed states J. Biol. Chem. 283 32848- 32859.
  • Smits, S.H. & L. Schmitt, (2008) Eine Strukturbiologie für Membranproteine. Nachrichten der Chemie: 24-27.
  • S. Jenewein & L. Schmitt (2007) Eine molekulare Analyse der ATP-Hydrolyse, BIOspektrum 1/2007 20-23.

  • N. Hanekop, M. Hoeing, L. Sohn-Boesser, M. Jebbar, L. Schmitt & E. Bremer, (2007) Crystal structure of the ligand-binding protein EhuB from Sinorhizobium meliloti reveals substrate recognition of the compatible solutes ectoine and hydroxyectoine, J. Mol. Biol. 374 1237-1250.

  • Kedrov, A., S. Wegmann, S.H. Smits, P. Goswami, H. Baumann & D.J. Muller, (2007) Detecting molecular interactions that stabilize, activate and guide ligand-binding of the sodium/proton antiporter MjNhaP1 from Methanococcus jannaschii. Journal of structural biology 159: 290-301.

  • C. Oswald, I. B. Holland & L. Schmitt (2006) Structural lessons from nucleotide-binding domains of ABC-transporters, Naunyn-Schmiedeberg´s Archives of Pharmacology, 372 385-399.

  • N. Hanekop, J. Zaitseva, S. Jenewein, I. B. Holland & L. Schmitt (2006) Molecular insights into the mechanism of ATP-hydrolysis by the NBD of the ABC-transporter HlyB, FEBS Lett. 580 1036-1041.

  • C. Horn, L. Sohn-Bösser, J. Breed, W. Welte, L. Schmitt & E. Bremer (2006) Molecular determinants for substrate specificity of the ligand-binding protein OpuAC from Bacillus subtilis for the compatible solutes glycine betaine and proline betaine, J. Mol. Biol., 357 592-606.

  • J. Zaitseva, C. Oswald, T. Jumpertz, S. Jenewein, A. Wiedenmann, I. B. Holland & L. Schmitt (2006) A structural analysis of asymmetry required for catalytic activity in an isolated ABC-ATPase domain dimer. EMBO J. 25 3432- 3443.

  • R. Ernst, J. Koch, C. Horn, R. Tampe & L. Schmitt (2006) Engineering ATPase activity in the isolated ABC-cassette of human TAP1. J. Biol. Chem. 281 27471- 27480.

  • B. Holland, L. Schmitt, & J. Young (2005) Type 1 protein secretion in bacteria: The ABC-transporter dependent pathway. Mol. Memb. Biol.22 29-39.

  • H. Benabdelhak, L. Schmitt, C. Horn, K. Jumel, M. A. Blight & I. B. Holland (2005) Positive co-operative activity and dimerization of the isolated ABC ATPase domain of HlyB from Escherichia coli Biochem. J. 368 1-7.

  • J. Zaitseva, S. Jenewein, T. Jumpertz, I. B. Holland, & L. Schmitt (2005) H662 is the linchpin in ATP hydrolysis of the nucleotide-binding domain of the ABC-transporter HlyB, EMBO J. 24 1901-1910.

  • J. Zaitseva, S. Jenewein, A. Wiedenmann, H. Benabdelhak, I. B. Holland, & L. Schmitt (2005) Functional characterization and ATP-induced dimerization of the isolated ABC-domain of the haemolysin B transporter. Biochemistry 44 9680-9690.

  • J. Zaitseva, S. Jenewein, C. Oswald, T. Jumpertz, I. B. Holland & L. Schmitt (2005) A molecular understanding of the catalytic cycle of the nucleotide-binding domain of the ABC transporter HlyB, Biochem. Soc. Trans., 33 990-995.

  • C. Horn, E. Bremer & L. Schmitt (2005) Functional overexpression and in vitro assembly of OpuA, an osmotically regulated ABC-transporter from Bacillus subtilis FEBS Lett., 579 5765-5768.

  • R. Ernst, R. Klemm, L. Schmitt, & K. Kuchler (2005) Yeast ABC-transporters – Cellular cleaning pumps Methods in Enzymol., 400 460-484.

  • C. Horn, S. Jenewein, L. Sohn-Bösser, E. Bremer & L. Schmitt (2005) Biochemical and structural analysis of the Bacillus subtilis ABC transporter OpuA and ist isolated subunits J. Mol. Memb. Biotechn., 10 76-91.

  • Vinothkumar, K.R., S.H. Smits & W. Kuhlbrandt, (2005) pH-induced structural change in a sodium/proton antiporter from Methanococcus jannaschii. The EMBO journal 24: 2720-2729.


  • J. Zaitseva, I. B. Holland & L. Schmitt (2004) The role of CAPS buffer in expanding the crystallzation space of the ATP-binding subunit of the ABC-transporter HlyB, Acta Cryst. D 60 1076-1084.

 

 

  • H. Benabdelhak, S. Kiontke, C. Horn, M. A. Blight, I. B. Holland & L. Schmitt (2003) A specific interaction of the NBD domain of the ABC-transporter HlyB and the C-terminal of its transport substrate, haemolysin A, J. Mol. Biol.327 1169-1179.

  • L. Schmitt, H. Benabdelhak, M. A. Blight, I. B. Holland & M. T. Stubbs (2003) Crystal structure of the NBD of the ABC-transporter HlyB: Identification of a variable region within the ABC helical domains, J. Mol. Biol. 330 333-343.

  • B. Holland, H. Benabdelhak, J. Young, A. de Lima Pimenta, L. Schmitt & M. A. Blight (2003) “Bacterial ABC-transporters involved in protein translocation in “ ABC proteins: from bacteria to man (eds. I. B. Holland, K. Kuchler, C. Higgins & S. Cole), Academic Press, London, 209-241.

  • C. Horn, E. Bremer & L. Schmitt (2003) Nucleotide dependent monomer/dimer equilibrium of OpuAA, the nucleotide binding protein of the osmotically regulated ABC-transporter OpuA from Bacillus subtilis, J. Mol. Biol. 334, 403-419.

  • S. Hutschenreiter, L. Neumann, U. Rädler, L. Schmitt & R. Tampé (2003) Metal-chelating amino acids as building block for synthetic receptors sensing of metal ions and histidine-tagged proteins ChemBioChem, 4 1340-1344.

  • L. Kranitz, H. Benabdelhak, C. Horn, M. A. Blight, I. B. Holland & L. Schmitt (2002) Crystallization and preliminary X-ray analysis of the ATP-binding domain of the ABC transporters haemolysin B from Escherichia coli. Acta Cryst. D 58 539-541.

  • L. Schmitt (2002) The first view of an ABC transporter: The X-ray crystal structure of MsbA from E. coli. ChemBioChem 3 161-165.

  • L. Schmitt & R. Tampé (2002) Structure and mechanism of ABC-transporters. Cur. Opin. Struct. Biol. 12 754-760.

  • Leonard, P.M., S.H. Smits, S.E. Sedelnikova, A.B. Brinkman, W.M. de Vos, J. van der Oost, D.W. Rice & J.B. Rafferty, (2001) Crystal structure of the Lrp-like transcriptional regulator from the archaeon Pyrococcus furiosus. The EMBO journal 20: 990-997.

  • Sedelnikova, S.E., S.H. Smits, P.M. Leonard, A.B. Brinkman, J. van der Oost, J.B. Rafferty & D.W. Rice, (2001) Crystallization and quaternary structure analysis of an Lrp-like regulatory protein from the hyperthermophile Pyrococcus furiosus. Acta crystallographica. Section D, Biological crystallography 57: 886-888.

  • L. Schmitt, C. Dietrich & R. Tampé (1994) Synthesis and characterization of chelator-lipids for reversible immobilization of engineered proteins at self-assembled lipid interfaces. J. Am. Chem. Soc. 116 8485-8491.

  • G. Brink, L. Schmitt, R. Tampé & E. Sackmann (1994) Self assembly of covalently anchored phospholipid suported membranes by use of DODA-Suc-NHS-lipids. Biochim. Biophys. Acta 1196 227-230.

  • L. Schmitt & R. Tampé (1995) Molecular recognition and organization of histidine-tagged biomolecules at self-assembled lipid interfaces using a novel class of chelator lipids.Prot. Engineering  8 121.

  • C. Dietrich, L. Schmitt & R. Tampé (1995) Molecular organization of histidine-tagged biomolecules at self-assembled lipid interfaces using a novel class of chelator lipids. Proc. Natl. Acad. Sci. USA 92 9014-9018.

  • S. Gritsch, K. Neumaier, L. Schmitt & R. Tampé (1995) Engineered fusion molecules at chelator lipid interfaces imaged by reflection interference contrast microscopy (RICM). Biosensors & Bioelectr. 10 805-812.

  • C. Dietrich, O. Boscheinen, K.-D. Scharf, L. Schmitt & R. Tampé (1996) Functional immobilization of a DNA-binding protein at a membrane interface via histidine-tag and synthetic chelator lipids. Biochemistry  35 1100-1105.

  • L. Schmitt, T. H. Bohanon, S. Denzinger, H. Ringsdorf & R. Tampé (1996) Specific protein docking to chelator lipid monolayers monitored by FT-IR spectroscopy at the air-water interface Angew. Chem. Int. Ed. Eng.  35 317-319.

  • R. Tampé, C. Dietrich, S. Gritsch, G. Elender & L. Schmitt (1996) Biofunctionalized membranes on solid surfaces  in „Nanofabrication and Biosystems“ (eds. H. C. Hoch, L. W. Jelinski & H. G. Craighead), Cambridge University Press, London.

  • L. Schmitt & R. Tampé (1996) ATP-lipids - Protein anchor and energy source in two dimensions J. Am. Chem. Soc. 118 5532-5543.

  • D. Wagner, U. G. Hofmann, L. Schmitt, I. Dorn, R. Tampé & H. E. Gaub (1997) Anomalous pH dependence of the coexistence pressure of the polymerizable two-chain lipid, N-methyl-(bis(pentacosadiinoyl)oxyethyl)amine. Eur. Biophys. J. 26 271-275.

  • L. Schmitt, J. J. Boniface, M. M. Davis & H. M. McConnell (1998) Kinetic isomers of a class II MHC-peptide complex. Biochemistry  37 17371-17380.

  • L. Schmitt, J. J. Boniface, M. M. Davis & H. M. McConnell (1999) Conformational isomers of a class II MHC-peptide complex in solution. J. Mol. Biol. 286 2409-2420.

  • N. H. Thomson, B. L. Smith, N. Almqvist, L. Schmitt, M. Kashlev, E. T. Kool & P. K. Hansma (1999) Oriented, active Escherichia coli RNA polymerase: An atomic force microscope study. Biophys. J. 76 1024-1033.

  • M. Ludwig, M. Rief, L. Schmitt, H. Li, F. Oesterhelt, M. Gautel & H. E. Gaub (1999) AFM, a tool for single-molecule experiments. Appl. Phys. A 68 173-176.

  • L. Schmitt, J. R. Kratz, M. M. Davis & H. M. McConnell (1999) Catalysis of peptide dissociation from class II MHC-peptide complexes. Proc. Natl. Acad. Sci. USA 96 6581-6586.

  • P. M. Kasson, J. D. Rabinowitz, L. Schmitt, M. M. Davis & H. M. McConnell (2000) Kinetics of peptide binding to the class II MHC protein I-Ek. Biochemistry 39 1048-1058.

  • L. Schmitt, M. Ludwig, H. E. Gaub  & R. Tampé (2000) A metal-chelating microscopy tip as a new toolbox for single molecule experiments by atomic force microscopy. Biophys. J. 78 3275-3285.

  • L. Schmitt & R. Tampé (2000) Affinity, specificity, diversity: A challenge for the ABC transporter TAP in cellular immunity. ChemBioChem 1 16-35.

List of patents

  • Agents and Methods for the Expression and Secretion of Peptides and Proteins; C. K. W. Schwarz & L. Schmitt, S. H. J. Smits, Europäisches Patentamt, 21.10.2011, EP11186225.6. Pending.
  • Methods for the Expression of Peptides and Proteins, C. K. W. Schwarz, S. Smits & L. Schmitt, Europäisches Patentamt, 17.04.2013, EP13164098.9. Pending.
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